Calibrating
Selecting the Standard Method (Standard Column)

Use the Standard method to determine how calibration is performed. Generally, a distinction is made between a calibration based on an internal or an external standard.

External means that the calibration is performed based on one or several standard samples. (This is the default.)

Using an internal standard substance means adding a standard to the unknown sample. This can be either before (External/Internal) or after (Internal) sample preparation. Either the standard (= Internal Standard) can be added to all samples or it can serve as a basis for a relative area calculation. In this case, results are displayed only in relation to the amount or area of the internal standard. (For more information, refer to Theory of Calibration,  Standard Methods.)

• In the Standard column, use the Standard Method for <Peak Name> dialog box to determine the standard method (External, Internal/External, Internal) for each peak to be calibrated. To open the dialog box, press the F8 key in the corresponding cell.

• For the last two options, at least one peak of the peak table must be used as the internal standard: In the Standard Method for <Peak Name> dialog box for the peak to be used, select Use this peak as Internal Standard (ISTD).

• Press the F8 key to enter the standard method assignment via an edit dialog box.